Quattro Premier LC/MS Resource Page
Intro
In EVE we have a Waters-Micromass Quattro Premier triple quadrupole mass spectrometer. This is designed for use with liquid sample inlet device, generally an HPLC or UPLC. For this reason, it is commonly referred to as an LC/MS (liquid chromotograph - mass spectrometer). This instrument is equipped with electrospray (ESI) and atmospheric pressure chemical ionization (APCI). Currently it is linked to our Acquity UPLC. There is also some useful documentation on the Quattro Micro page.
Useful Documents
- The EWRE Easy Guide to LC/MS
- Instrument (Quattro Premier)
- Operator's Guide (Table of Contents); see below for contents:
| Chapt | Topic | pp. | Append. | Topic | pp. | |
|---|---|---|---|---|---|---|
| 1 | Inst. Description | 1-16 | A | Startup/shutdown | 225-229 | |
| 2 | ESI | 17-34 | B | syringe pump | 231-232 | |
| 3 | APCI | 35-52 | C | Tune Window | 233-249 | |
| 4 | Multi-Mode | 53-55 | D | Calibration | 251-256 | |
| 5 | Data Acquistion | 57-111 | E | Specs | 257-258 | |
| 6 | Mass Calibration | 113-132 | F | Theory | 259-268 | |
| 7 | Maintenance | 133-209 | G | Solvents | 269-270 | |
| 8 | Troubleshooting | 211-224 | index | 271-279 |
- Software
Safety; OG Addendum , from Waters Corp.
- Peripherals
- Edwards Pump Manual; from the manufacturer
Relevant Methods and SOPs
- LC/MS Basics and General Literature
- Solid Pase Extraction
- EDCs & PPCPs
- UMass SOP
- General Literature on Methods for PPCPs
- Nucleic Acids
- Amino Acids
Unit Identifiers
Component |
Name | Model # | Ser# | Manufacturer |
|---|---|---|---|---|
Mass Spectrometer |
Quattro Premier | Waters Corp. | ||
Vacuum Pump |
E2M28 | A373- | BOC Edwards | |
Controlling Software |
MassLynx | Version 4.0 |
In-House Expertise
- Users: Soon-Mi Kim, Kun Xiang, Varun Srinivasan, Stephanie Zimmers
- Special Consultant: Larry Kramer