Quattro Micro LC/MS Resource Page

Intro

In EVE we have a Waters-Micromass Quattro Micro triple quadrupole mass spectrometer. This is designed for use with liquid sample inlet device, generally an HPLC or UPLC. For this reason, it is commonly referred to as an LC/MS (liquid chromotograph - mass spectrometer). This instrument is equipped with electrospray (ESI) and atmospheric pressure chemical ionization (APCI). Currently it is linked to one of our Alliance HPLCs. There is also some useful documentation on the Quattro Premier page.

Useful Documents

• Instrument
  • Quattro Micro Easy Operating Guide: prepared by UMass EVE operators
  • Quattro Micro Operating Manual: from Waters Corp, the ultimate authority
  • Quattro Micro Fundamentals: from Waters Corp.
  • QM Training Slides: #1, #2, #3, #4, #5
• Software
• Mass Lynx 4.0 Manual
• Peripherals
• Edwards Pump Manual; from the manufacturer

Relevant Methods and SOPs

• LC/MS Basics and General Literature
• Solid Phase Extraction
• Amino Acids

Unit Identifiers

In-House Expertise

• Users: Varun Srinivasan, Boning Liu
• Special Consultant: Larry Kramer